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R&D Systems recombinant human il1β il 1f2 protein
Recombinant Human Il1β Il 1f2 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+il1%CE%B2+il+1f2+protein/pm41922353-268-19-27?v=R%26D+Systems
Average 96 stars, based on 337 article reviews

recombinant human il1β il 1f2 protein - by Bioz Stars, 2026-07

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Effect of curcumin nanoemulsion on cell viability and chemotactic migration of macrophages towards IL1β-induced chondrocytes. ( A , B ) Cell viability by CHON-001 and RAW 264.7 cells, respectively, exposed to different concentrations of curcumin and nanoemulsion (0.1–100 μM) for 24 h. Cell viability was evaluated using the MTT assay and calculated by comparing optical density values between the control and treatment groups after normalizing them with blank values, and expressed as a percentage of the control. Each value is represented as mean ± SEM ( n = 8). ( C ) Transwell co-culture model. ( D ) Representative images of calcein AM (green)-stained macrophages on the underside of 8 μm insert after 24 h of co-culture. Chondrocytes were cultured in the lower chamber and exposed to stimulus, while macrophages were incubated in the insert with plain assay media. Images (magnification, 10×; scale bar, 100 μm) represent macrophage migration towards ( i ) unstimulated chondrocytes, ( ii ) IL1β-induced chondrocytes, ( iii ) IL1β-induced chondrocytes treated with curcumin (10 μM), and ( iv ) IL1β-induced chondrocytes treated with nanoemulsion (10 μM). ( E ) Quantification of migrated cells using mean fluorescence intensity per unit area. Data were analyzed using one-way ANOVA with Tukey’s multiple comparisons, and p < 0.05 was considered statistically significant compared to the control. Unlike letters denote significant differences.

Journal: International Journal of Molecular Sciences

Article Title: Intra-Articular Delivery of Nanoemulsified Curcumin Ameliorates Joint Degeneration in a Chemically Induced Model of Osteoarthritis

doi: 10.3390/ijms262211212

Figure Lengend Snippet: Effect of curcumin nanoemulsion on cell viability and chemotactic migration of macrophages towards IL1β-induced chondrocytes. ( A , B ) Cell viability by CHON-001 and RAW 264.7 cells, respectively, exposed to different concentrations of curcumin and nanoemulsion (0.1–100 μM) for 24 h. Cell viability was evaluated using the MTT assay and calculated by comparing optical density values between the control and treatment groups after normalizing them with blank values, and expressed as a percentage of the control. Each value is represented as mean ± SEM ( n = 8). ( C ) Transwell co-culture model. ( D ) Representative images of calcein AM (green)-stained macrophages on the underside of 8 μm insert after 24 h of co-culture. Chondrocytes were cultured in the lower chamber and exposed to stimulus, while macrophages were incubated in the insert with plain assay media. Images (magnification, 10×; scale bar, 100 μm) represent macrophage migration towards ( i ) unstimulated chondrocytes, ( ii ) IL1β-induced chondrocytes, ( iii ) IL1β-induced chondrocytes treated with curcumin (10 μM), and ( iv ) IL1β-induced chondrocytes treated with nanoemulsion (10 μM). ( E ) Quantification of migrated cells using mean fluorescence intensity per unit area. Data were analyzed using one-way ANOVA with Tukey’s multiple comparisons, and p < 0.05 was considered statistically significant compared to the control. Unlike letters denote significant differences.

Article Snippet: IL1β (#201-LB-005) was procured from R&D Systems (Minneapolis, MN, USA).

Techniques: Migration, MTT Assay, Control, Co-Culture Assay, Staining, Cell Culture, Incubation, Fluorescence

Expression of inflammatory mediator NF-κB in RAW 264.7 macrophages following IL1β stimulation in the presence of curcumin/nanocurcumin (5–10 μM). ( A ) Immunoblot of NF-κB and ( B ) its relative protein expression normalized with β-actin (mean ± SEM). ( C ) Nuclear and cytosolic expression of NF-κB in RAW 264.7 macrophages using immunocytochemistry after treating with IL1β and curcumin nanoemulsion (10 μM). Cells were stained with Alexa Fluor 594-tagged NF-κB (red) and DAPI (blue) to stain nuclei with original magnification of 40×; scale bar 100 μm. Images were captured using a confocal laser scanning microscope with an oil immersion objective (TCS SP5, Leica Microsystems, Germany). ( D ) Corrected total cell fluorescence (CTCF) was calculated, analyzed statistically and expressed in arbitrary units [CTCF = integrated density—(area of selected cell × mean fluorescence of background readings)]. Data were analyzed using one-way ANOVA with post hoc Tukey’s multiple comparison test, with p < 0.05 considered significant, and results depicted using different letters.

Journal: International Journal of Molecular Sciences

Article Title: Intra-Articular Delivery of Nanoemulsified Curcumin Ameliorates Joint Degeneration in a Chemically Induced Model of Osteoarthritis

doi: 10.3390/ijms262211212

Figure Lengend Snippet: Expression of inflammatory mediator NF-κB in RAW 264.7 macrophages following IL1β stimulation in the presence of curcumin/nanocurcumin (5–10 μM). ( A ) Immunoblot of NF-κB and ( B ) its relative protein expression normalized with β-actin (mean ± SEM). ( C ) Nuclear and cytosolic expression of NF-κB in RAW 264.7 macrophages using immunocytochemistry after treating with IL1β and curcumin nanoemulsion (10 μM). Cells were stained with Alexa Fluor 594-tagged NF-κB (red) and DAPI (blue) to stain nuclei with original magnification of 40×; scale bar 100 μm. Images were captured using a confocal laser scanning microscope with an oil immersion objective (TCS SP5, Leica Microsystems, Germany). ( D ) Corrected total cell fluorescence (CTCF) was calculated, analyzed statistically and expressed in arbitrary units [CTCF = integrated density—(area of selected cell × mean fluorescence of background readings)]. Data were analyzed using one-way ANOVA with post hoc Tukey’s multiple comparison test, with p < 0.05 considered significant, and results depicted using different letters.

Article Snippet: IL1β (#201-LB-005) was procured from R&D Systems (Minneapolis, MN, USA).

Techniques: Expressing, Western Blot, Immunocytochemistry, Staining, Laser-Scanning Microscopy, Fluorescence, Comparison

All cells were subjected to serum starvation prior to treatment. a Representative images of the human keloid-derived fibroblast KB-AA35 (derived from a 35-year-old female African–American donor; Supplementary Table ) stained with α-smooth muscle actin (α-SMA) by immunofluorescence staining. Scale bar, 25 μm. b Expression of ACTA2 in FB-AA36 fibroblasts and KB-AA35 fibroblasts. Data were normalized to the ACTA2 level of FB-AA36 fibroblasts. c Quantification of KB-AA35 fibroblast apoptosis by flow cytometry with Annexin V-FITC and PI staining. d Expression of IL1B in KB-AA35 fibroblasts with or without FMOD treatment. Data were normalized to the IL1B level without FMOD treatment. e Active IL1β production in KB-AA35 fibroblasts with or without FMOD treatment. Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P- values were determined by two-tailed unpaired t -tests ( b – e ). * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: All cells were subjected to serum starvation prior to treatment. a Representative images of the human keloid-derived fibroblast KB-AA35 (derived from a 35-year-old female African–American donor; Supplementary Table ) stained with α-smooth muscle actin (α-SMA) by immunofluorescence staining. Scale bar, 25 μm. b Expression of ACTA2 in FB-AA36 fibroblasts and KB-AA35 fibroblasts. Data were normalized to the ACTA2 level of FB-AA36 fibroblasts. c Quantification of KB-AA35 fibroblast apoptosis by flow cytometry with Annexin V-FITC and PI staining. d Expression of IL1B in KB-AA35 fibroblasts with or without FMOD treatment. Data were normalized to the IL1B level without FMOD treatment. e Active IL1β production in KB-AA35 fibroblasts with or without FMOD treatment. Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P- values were determined by two-tailed unpaired t -tests ( b – e ). * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Article Snippet: His-tag-free 100 μg recombinant human IL1β (SDS-PAGE: 17 kDa, carrier-free; 201-LB-010/CF, R&D Systems), 100 μg IL1R1 (SDS-PAGE: 55 kDa, carrier-free; 269-1R-100/CF, R&D Systems), or 100 μg IL1RAP (SDS-PAGE: 70–75 kDa, carrier-free; 676-CP-100/CF, R&D Systems) were used as prey, respectively to assay their interaction with FMOD separately.

Techniques: Derivative Assay, Staining, Immunofluorescence, Expressing, Flow Cytometry, Two Tailed Test

All cells were subjected to serum starvation prior to treatment. a Representative images of normal skin (from abdomen), keloid (from abdomen), and hypertrophic scar (from left shoulder) tissues collected from the donor with the National Disease Research Interchange (NDRI) reference ID 1914138 with α-smooth muscle actin (α-SMA) staining by immunohistochemistry (IHC; upper), and the representative images of dermal fibroblasts isolated from these tissues with α-SMA staining by immunofluorescence staining (lower). b Expression of ACTA2 in dermal fibroblasts derived from tissues described from ( a ). Data were normalized to the ACTA2 level of fibroblasts isolated from the normal skin. c Representative flow cytometry plots of fibroblasts derived from tissues described in ( a ) with Annexin V-FITC and PI staining. d Quantification of apoptotic dermal fibroblasts by flow cytometry from ( c ). e The influence of FMOD on cell apoptosis with and without IL1β was calculated from ( d ). Scale bars, 50 μm (black) or 25 μm (white). Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P -values were determined by two-tailed unpaired t -tests ( b – e ). N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: All cells were subjected to serum starvation prior to treatment. a Representative images of normal skin (from abdomen), keloid (from abdomen), and hypertrophic scar (from left shoulder) tissues collected from the donor with the National Disease Research Interchange (NDRI) reference ID 1914138 with α-smooth muscle actin (α-SMA) staining by immunohistochemistry (IHC; upper), and the representative images of dermal fibroblasts isolated from these tissues with α-SMA staining by immunofluorescence staining (lower). b Expression of ACTA2 in dermal fibroblasts derived from tissues described from ( a ). Data were normalized to the ACTA2 level of fibroblasts isolated from the normal skin. c Representative flow cytometry plots of fibroblasts derived from tissues described in ( a ) with Annexin V-FITC and PI staining. d Quantification of apoptotic dermal fibroblasts by flow cytometry from ( c ). e The influence of FMOD on cell apoptosis with and without IL1β was calculated from ( d ). Scale bars, 50 μm (black) or 25 μm (white). Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P -values were determined by two-tailed unpaired t -tests ( b – e ). N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Techniques: Staining, Immunohistochemistry, Isolation, Immunofluorescence, Expressing, Derivative Assay, Flow Cytometry, Two Tailed Test

All cells were subjected to serum starvation prior to treatment. a Representative plots of wildtype (WT) and IL1B -/- BJ-myofibroblasts stained with Annexin V-allophycocyanin (APC) and DAPI staining. b Quantification of BJ-myofibroblast apoptosis from ( a ). c Impacts of fibromodulin (FMOD) and IL1β on cell apoptosis determined from ( b ). Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P- values were determined by two-tailed unpaired t -tests ( b , c ). N.S ., not significant, P > 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: All cells were subjected to serum starvation prior to treatment. a Representative plots of wildtype (WT) and IL1B -/- BJ-myofibroblasts stained with Annexin V-allophycocyanin (APC) and DAPI staining. b Quantification of BJ-myofibroblast apoptosis from ( a ). c Impacts of fibromodulin (FMOD) and IL1β on cell apoptosis determined from ( b ). Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P- values were determined by two-tailed unpaired t -tests ( b , c ). N.S ., not significant, P > 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Techniques: Staining, Two Tailed Test

All cells were subjected to serum starvation prior to treatment. a ELISA assessment of active IL1β production from the IL1B- , IL1R1- , and IL1RAP- knockdown BJ-myofibroblasts, as well as the control cells transfected with non-targeting siRNA control, respectively. b Impacts of FMOD on active IL1β production determined from ( a ). c Representative flow cytometry plots of BJ-myofibroblasts described in ( a ). Cell apoptosis was evaluated with staining of Annexin V-FITC and PI staining. d Quantification of apoptotic BJ-myofibroblasts by flow cytometry from ( c ). e Impacts of FMOD on BJ-myofibroblast apoptosis determined from ( d ). f Quantification of pyroptotic BJ-myofibroblasts by flow cytometry from ( c ). g Impacts of FMOD on BJ-myofibroblast pyroptosis determined from ( f ). Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P -values were determined by two-tailed unpaired t -tests ( a – h ). N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: All cells were subjected to serum starvation prior to treatment. a ELISA assessment of active IL1β production from the IL1B- , IL1R1- , and IL1RAP- knockdown BJ-myofibroblasts, as well as the control cells transfected with non-targeting siRNA control, respectively. b Impacts of FMOD on active IL1β production determined from ( a ). c Representative flow cytometry plots of BJ-myofibroblasts described in ( a ). Cell apoptosis was evaluated with staining of Annexin V-FITC and PI staining. d Quantification of apoptotic BJ-myofibroblasts by flow cytometry from ( c ). e Impacts of FMOD on BJ-myofibroblast apoptosis determined from ( d ). f Quantification of pyroptotic BJ-myofibroblasts by flow cytometry from ( c ). g Impacts of FMOD on BJ-myofibroblast pyroptosis determined from ( f ). Data presented as mean ± s.d. overlaying all the data points. N = 3 biological replicates; P -values were determined by two-tailed unpaired t -tests ( a – h ). N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Techniques: Enzyme-linked Immunosorbent Assay, Knockdown, Control, Transfection, Flow Cytometry, Staining, Two Tailed Test

a Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein (M.P.) extracted from BJ-myofibroblasts. Blot is representative of N = 2 biological replicates ( a – e ). f Surface plasmon resonance (SPR) spectrum characterizing the binding properties between IL1R1 and FMOD. g SPR spectrum characterizing the binding properties between IL1RAP and FMOD. h In silico analysis of protein-protein interactions between FMOD with IL1β-IL1R1 binary complex. i . In silico analysis of protein-protein interactions between FMOD and IL1β-IL1R1-IL1RAP ternary complex. j In silico analysis of binding energy among IL1β-IL1R1-IL1RAP ternary complex components. k In silico analysis of the average distance of the involved amino acid residues of IL1β-IL1R1-IL1RAP ternary complex. More details about the amino acid residue interactions are demonstrated in Supplementary Fig. . Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: a Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein (M.P.) extracted from BJ-myofibroblasts. Blot is representative of N = 2 biological replicates ( a – e ). f Surface plasmon resonance (SPR) spectrum characterizing the binding properties between IL1R1 and FMOD. g SPR spectrum characterizing the binding properties between IL1RAP and FMOD. h In silico analysis of protein-protein interactions between FMOD with IL1β-IL1R1 binary complex. i . In silico analysis of protein-protein interactions between FMOD and IL1β-IL1R1-IL1RAP ternary complex. j In silico analysis of binding energy among IL1β-IL1R1-IL1RAP ternary complex components. k In silico analysis of the average distance of the involved amino acid residues of IL1β-IL1R1-IL1RAP ternary complex. More details about the amino acid residue interactions are demonstrated in Supplementary Fig. . Source data are provided as a Source Data file.

Techniques: Pull Down Assay, Recombinant, Incubation, In Vitro, Membrane, SPR Assay, Binding Assay, In Silico, Protein-Protein interactions, Residue

All cells were subjected to serum starvation prior to treatment. a Representative images of in situ IL1R1:IL1β-proximity ligation assay (PLA) staining on attached BJ-myofibroblasts. Phalloindin was used to stain F-actin, while DAPI was used for nuclei staining. b – g Quantification of IL1R1:IL1β-PLA + signal per cell at 1- ( b ), 6- ( c ), 12- ( d ), 24- ( e ), 48- ( f ), and 72-h ( g ) from ( a ). h Representative flow cytometry plot and quantification of IL1R1:IL1β-PLA staining of BJ-myofibroblasts at 24 h post-treatment. Scale bar, 50 μm. Data presented as violin plots ( b-g ) or mean ± s.d. h overlaying all the data points. N = 10 ( a – g ) or 3 ( h ), respectively; P -values were determined by two-tailed Mann–Whitney U tests ( b – g ) or two-tailed unpaired t -tests ( h ), respectively. N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: All cells were subjected to serum starvation prior to treatment. a Representative images of in situ IL1R1:IL1β-proximity ligation assay (PLA) staining on attached BJ-myofibroblasts. Phalloindin was used to stain F-actin, while DAPI was used for nuclei staining. b – g Quantification of IL1R1:IL1β-PLA + signal per cell at 1- ( b ), 6- ( c ), 12- ( d ), 24- ( e ), 48- ( f ), and 72-h ( g ) from ( a ). h Representative flow cytometry plot and quantification of IL1R1:IL1β-PLA staining of BJ-myofibroblasts at 24 h post-treatment. Scale bar, 50 μm. Data presented as violin plots ( b-g ) or mean ± s.d. h overlaying all the data points. N = 10 ( a – g ) or 3 ( h ), respectively; P -values were determined by two-tailed Mann–Whitney U tests ( b – g ) or two-tailed unpaired t -tests ( h ), respectively. N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Techniques: In Situ, Proximity Ligation Assay, Staining, Flow Cytometry, Two Tailed Test, MANN-WHITNEY

All cells were subjected to serum starvation prior to treatment. a Representative images of in situ IL1R1:IL1RAP-proximity ligation assay (PLA) staining on attached BJ-myofibroblasts. Phalloindin was used to stain F-actin, while DAPI was used for nuclei staining. b – g Quantification of IL1R1:IL1RAP-PLA + signal per cell at 1- ( b ), 6- ( c ), 12- ( d ), 24- ( e ), 48- ( f ), and 72-h ( g ) after the treatment of FMOD and/or IL1β from ( a ). h Representative flow cytometry plot and quantification of IL1R1:IL1RAP-PLA staining of BJ-myofibroblasts at 24 h post-treatment. Scale bar, 50 μm. Data presented as violin plots ( b – g ) or mean ± s.d. ( h ) overlaying all the data points. N = 10 ( a – g ) or 3 ( h ), respectively; P -values were determined by two-tailed Mann-Whitney U tests ( b – g ) or two-tailed unpaired t -tests ( h ). N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: All cells were subjected to serum starvation prior to treatment. a Representative images of in situ IL1R1:IL1RAP-proximity ligation assay (PLA) staining on attached BJ-myofibroblasts. Phalloindin was used to stain F-actin, while DAPI was used for nuclei staining. b – g Quantification of IL1R1:IL1RAP-PLA + signal per cell at 1- ( b ), 6- ( c ), 12- ( d ), 24- ( e ), 48- ( f ), and 72-h ( g ) after the treatment of FMOD and/or IL1β from ( a ). h Representative flow cytometry plot and quantification of IL1R1:IL1RAP-PLA staining of BJ-myofibroblasts at 24 h post-treatment. Scale bar, 50 μm. Data presented as violin plots ( b – g ) or mean ± s.d. ( h ) overlaying all the data points. N = 10 ( a – g ) or 3 ( h ), respectively; P -values were determined by two-tailed Mann-Whitney U tests ( b – g ) or two-tailed unpaired t -tests ( h ). N.S ., not significant, P > 0.05; * P < 0.05; ** P < 0.005. Source data are provided as a Source Data file.

Techniques: In Situ, Proximity Ligation Assay, Staining, Flow Cytometry, Two Tailed Test, MANN-WHITNEY

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